Prostate cancer (in-vitro) cell culture. LNCaP cells are a cell line of human cells commonly used in the field of oncology. LNCaP cells are androgen-sensitive and derive from a human prostate adenocarcinoma (lymph node metastasis) of a 50-year-old caucasian male patient. They are adherent epithelial cells growing in aggregates and as single cells. Prostate cancer is the most frequently diagnosed cancer in men. One major obstacle in prostate cancer research is the lack of cell lines that closely mimic human disease progression. Two hallmarks of metastatic human prostate cancer include the shift of aggressive PCa from androgen-sensitivity to an androgen insensitive state, and the preference of primary prostate cancer cells to metastasize to bone. Although the generation of androgen insensitive cell lines has been demonstrated in “classic” cell lines (DU145 and PC3), the behavior of these cells in bone metastasis does not fully mimic clinical human disease. Therefore, LNCaP sublines have been generated to develop an androgen insensitive prostate cancer cell model that more closely mimics clinical disease.
Human in-vitro Cell Culture
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